2Department of Heart Center, Faculty of Medicine, Tehran University of Medical Sciences, Tehran-Iran
3Department of Medical Mycology and Parasitology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran-Iran
4Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran-Iran; Department of Medical Genetics, University of Alberta, Edmonton, AB-Canada.
Abstract
Objective: It is suggested that C771G (His241Gln) polymorphism of MLXIPL gene might be a genetic risk factor for coronary artery disease (CAD); therefore, the aim of the present study was to investigate the association between C771G polymorphism of MLXIPL gene and the pathogenesis of CAD in Iranian patients with coronary artery stenosis and control subjects.
Materials and Methods: Two hundred and five patients with coronary artery stenosis and 195 healthy control subjects were included in this study. MLXIPL genotypes were determined by polymerase chain reaction and restriction fragment length polymorphism (RFLP).
Results: There was an association between the MLXIPL polymorphism and quantitative lipid traits in patient group. Distribution of the CC genotype of MLXIPL was more frequent in patients, (?2=5.13; p<0.005) and after adjustment for classical CAD risk factors, the MLXIPL CC genotype was independently associated with CAD (OR=1.98, 95% CI, 1.12-4.11; p=0.02). Distribution of MLXIPL genotypes were significantly different as compared with the severity of stenosis (?2=6.34; p<0.05).
Conclusion: These results suggest that C771G polymorphism of MLXIPL gene is associated with stenosis and its severity.
2Department of Heart Center, Faculty of Medicine, Tehran University of Medical Sciences, Tehran-Iran
3Department of Medical Mycology and Parasitology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran-Iran
4Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran-Iran; Department of Medical Genetics, University of Alberta, Edmonton, AB-Canada.
Amaç: MLXIPL geninin C771G (His 241 Gln) polimorfizminin koroner arter hastlığı (KAH) için genetik bir risk faktörü olabileceği ileri sürülüyor; bundan dolayı, sunulan çalışmanın amacı, İranlı koroner arter darlığı olan ve kontrol kişilerinde KAH patogenezi ile, MLXIPL genini C//G polimorfizmi ilişkisini araştırmaktadır.
Yöntemler: Koroner arter darlığı 205 hasta ile 125 sağlıklı kontrol birey bu çalışmaya dahil edildi. MLXIPL genetipleri polimeraz zincir reaksiyonu ve kısıtlamalı fragman uzunluk polimorfizmi ile tayin edildi.
Bulgular: MLXIPL polimorfizmi ve kantitatif lipid özellikli hasta grubu arasında bir ilişki vardı. MLXIPL’nin CC genotipinin dağılımı hastalarda daha sıktı, (x2=5,13; p<0,005) ve kronik KAH risk faktörlerine göre ayarlama yapıldıktan sonra MLXIPL CC genotipi KAH ile bağımsız bir ilişki gösteriyordu (OR=1,98, %95 CI, 1.12-4,11; p=0,02) MLXIPL genotiplerinin dağılımı darlığın şiddeti ile mukayese edildiğinde anlamlı farklılık gösterdi (x2=6,34; p<0,05).
Sonuçlar: Bu bulgular MLXIPL geninin C771G polimorfizminin darlık ve onun şiddeti ile ilişkili olduğunu düşündürmektedir.