Objective: We aimed to study the change in the plasma homocysteine concentration in the early stage of acute myocardial infarction and its relationship with the acute phase reactants. Materials and Methods: We included into the study 33 patients who were admitted to the hospital with acute myocardial infarction within the first three hours after the onset of symptoms. The plasma samples were obtained on admission (within 3 hours onset of symptom) and at 6, 12, 24 hours and 2, 4, 7, 30 and 90th day after admission. Results: The serial homocysteine measurements were as following: 11.87±0.71 µmol/L, 11.89±0.62 µmol/L, 11.37±0.83 µmol/L, 10.96±0.93 µmol/L, 11.37 ±0.89 µmol/L, 11.24±0.66 µmol/L, 13.09±0.64 µmol/L, 12.85±0.71 µmol/L and 12.19±0.91 µmol/L respectively (p=0.05). Statistically significant difference was found only between the hour 24 and the day 7 (p=0.04). However, there was no statistically significant difference between the admission level and none of the other time points. No correlation was identified between acute phase reactants and lipid parameters that were measured serially at the same time periods and homocysteine levels. Conclusion: Although homocysteine plasma values obtained during the sixth and twelfth hours of acute myocardial infarction provide reliable results as a risk markers, timing of blood sampling during the myocardial infarction does not have significant role since plasma values of homocysteine did not affect acute phase reactants.