2Harran Üniversitesi Tıp Fakültesi, Kardiyoloji Anabilim Dalı, Şanlıurfa, Türkiye
3From Department of Clinical Chemistry Faculty of Medicine, Harran University, Şanlıurfa, Turkey
Abstract
Objective: We investigated the acute effects of treadmill exercise test (TET) on total peroxide, total antioxidant capacity (TAC), oxidative stress index (OSI) and DNA damage levels in voluntary and untrained healthy subjects. Materials and Methods: A total of 113 untrained healthy subjects were included in the study. All subjects maintained a similar diet and physical activi- ty for a week before the test. Blood samples were obtained before and after TET. Total peroxide, TAC, vitamin C and DNA damage were measured. The DNA damage was analyzed by using the Comet assay and OSI was calculated using total peroxide and TAC values. Results: Treadmill exercise test leads to the increase of total peroxide (12 ± 3 μmol H2O2/L to 14 ± 3 μmol H2O2/L, p<0.001), OSI (0.72 ± 0.18 AU to 0.81 ± 0.22 AU, p<0.001), and to the decrease of TAC (1.78 ± 0.16 mmol Trolox Eq./L to 1.72 ± 0.15 mmol Trolox Eq./L, p<0.001) and vit- amin C levels (98 ± 4.2 μmol/L to 95 ± 3.4 μmol/L, p<0.001). There was not significant difference in DNA damage. Conclusion: Our findings demonstrate that TET increases oxidants, decreases TAC and vitamin C namely, the balance shift towards oxida- tive side, but this stress is not enough to produce DNA damage.