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microRNA-21 abrogates palmitate-induced cardiomyocyte apoptosis through caspase-3/NF-kB signal pathways [Anatol J Cardiol]
Anatol J Cardiol. Ahead of Print: AJC-03604 | DOI: 10.14744/AnatolJCardiol.2018.03604  

microRNA-21 abrogates palmitate-induced cardiomyocyte apoptosis through caspase-3/NF-kB signal pathways

Xiaodi Zhou1, Bo Chang2, Yuzhong Gu2
1Department Of Emergency Pediatrics, Affiliated Hospital Of Nantong University, Nantong, China
2Department Of Cardiology, The Sixth People‘s Hospital Of Nantong, Nantong, China

Objective: The aim of the study was to investigate the role of microRNA-21 (miR-21) in cardiomyocyte apoptosis and to determine the possible mechanism.
Methods: H9c2 embryonic rat heart-derived cells were used in the study. Cell viability was determined by MTT assay and flow cytometry was used to evaluate cell apoptosis. Reverse transcription polymerase chain reaction (RT-PCR) and western blot assays were used to detect mRNA and protein expression of apoptosis-related proteins and miR-21. ELISA was used to detect reactive oxygen species (ROS).
Results: Palmitate exposure greatly reduced miR-21 expression in cardiomyocytes. Apoptosis increased when miR-21 was inhibited with or without palmitate exposure. Consistently, reduced apoptosis was observed when miR-21 was overexpressed in cardiomyocytes. Caspase-3 activity was reduced after palmitate exposure. Bcl-2 protein expression was increased in H9c2 cells when transfected with the miR-21 mimic. MiR-21 overexpression alone did not induce ROS or DNA fragmentation; however, in conjunction with palmitate exposure, mimic miR-21 reduced ROS and DNA fragmentation. Moreover, palmitate administration overcame the antioxidant effect of 3 mM N-acetylcysteine (NAC) to significantly inhibit apoptosis, DNA fragmentation, and caspase-3 activity. Exposure to palmitate greatly reduced p65 and p-p38 expression in the nucleus. A p38 inhibitor had no effect on the expression of Bcl-2 and cleaved caspase-3 in H9c2 cells alone; however, when combined with exposure to palmitate the p38 inhibitor induced Bcl-2 expression and inhibited caspase-3 activity. The p38 inhibitor by itself did not induce apoptosis, ROS production, or DNA fragmentation in H9c2 cells, but when palmitate was included with the p38 inhibitor, apoptosis, ROS production, and DNA fragmentation were reduced.
Conclusion: miR-21 protects cardiomyocytes from apoptosis that is induced by palmitate through the caspase-3/NF-kB signal pathways.

Keywords: miR-21, palmitate-induced apoptosis, cardiomyocyte, caspase-3/NF-kB signal pathways




Corresponding Author: Yuzhong Gu, China


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