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Combination of PBMC miR-19b-5p, miR-221, miR-25-5p and hypertension correlates with increased risk of heart failure in coronary heart disease patients [Anatol J Cardiol]
Anatol J Cardiol. Ahead of Print: AJC-43255 | DOI: 10.14744/AnatolJCardiol.2018.43255  

Combination of PBMC miR-19b-5p, miR-221, miR-25-5p and hypertension correlates with increased risk of heart failure in coronary heart disease patients

Yuan Yao1, Tao Song1, Gang Xiong2, Zhaogui Wu1, Qi Li1, Hao Xia1, Xuejun Jiang1
1Department of Cardiology, Renmin Hospital of Wuhan University, Hubei General Hospital; Cardiovascular Research Institute of Wuhan University, Hubei Key Laboratory of Cardiology, Wuhan, China
2Department of Cardiology, Wuhan Asia Heart Hospital, Wuhan, China

Objective: The aim of this study was to explore the difference of miRNA profiles in peripheral blood mononuclear cells (PBMC) between coronary heart disease (CHD) patients with heart failure (HF) and CHD patients without HF, and to assess the values of differentially expressed miRNAs (DEMs) for the risk of HF in CHD patients.
Methods: Six CHD patients with HF and 6 age and gender-matched CHD patients without HF were enrolled in the exploration stage, and 44 CHD patients with HF and 42 age and gender-matched CHD patients without HF were recruited in the validation stage. Peripheral blood samples were collected from all participants and PBMC were separated for miRNA detection. microRNA microarray and Quantitative polymerase chain reaction (qPCR) were performed to assess the miRNA expression.
Results: In the exploration stage, heat map analysis showed that CHD patients with HF could be distinguished from CHD patients without HF by PMBC miRNA expressions, and 63 down-regulated DEMs and 84 upregulated DEMs in PBMC were identified in CHD patients with HF by volcano map, and top 8 DEMs were selected by their P values. In the validation stage, PBMC miR-221, miR-19b-5p and miR-25-5p were found to be markedly dysregulated in CHD patients with HF. Multiple logistic regression displayed that PBMC miR-221, miR-19b-5p, miR-25-5p and hypertension were independent predictive factors for HF in CHD patients. Roc curve demonstrated that the AUC of the combination of miR-221, miR-19b-5p, miR-25-5p and hypertension was 0.871 (95% CI: 0.794-0.944).
Conclusion: CHD patients with HF could be differentiated from CHD patients without HF according to PBMC miRNA profiles, and the combination of PBMC miR-19b-5p, miR-221, miR-25-5p as well as hypertension correlates with increased risk of HF in CHD patients.

Keywords: miRNA, profile, risk, heart failure, coronary heart disease




Corresponding Author: Xuejun Jiang, China


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