Effects of administration of omega-3 fatty acids with or without vitamin E supplementation on adiponectin gene expression in PBMCs and serum adiponectin and adipocyte fatty acid-binding protein levels in male patients with CAD

Ramezani, Atena; Koohdani, Fariba; Djazayeri, Abolghasem; Nematipour, Ebrahim; Ali  Keshavarz, Seyed; Saboor-Yaraghi, Ali-Akbar; Eshraghian, Mohammad-Reza; Yousefinejad, Abbas; Javanbakht, Hassan; Zarei, Mahnaz; Gholamhosseini, Sanaz; Djalali, Mahmoud

doi:10.5152/akd.2015.5849

Original Investigation

Effects of administration of omega-3 fatty acids with or without vitamin E supplementation on adiponectin gene expression in PBMCs and serum adiponectin and adipocyte fatty acid-binding protein levels in male patients with CAD

Atena Ramezani ¹ , Fariba Koohdani ¹ , Abolghasem Djazayeri ² , Ebrahim Nematipour ³ , Seyed Ali Keshavarz ⁴ , Ali-Akbar Saboor-Yaraghi ¹ , Mohammad-Reza Eshraghian ⁵ , Abbas Yousefinejad ¹ , Hassan Javanbakht ¹ , Mahnaz Zarei ¹ , Sanaz Gholamhosseini ¹ , Mahmoud Djalali ¹

¹Department of Molecular and Cellular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences; Tehran-Iran
²Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences; Tehran-Iran
³Department of Cardiology, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences; Tehran-Iran
⁴Department of Clinical Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences; Tehran-Iran
⁵Department of Biostatistics, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences; Tehran-Iran

Anatol J Cardiol 2015; 15(12): 981-989 PubMed ID: 25880054 PMCID: 5368470 DOI: 10.5152/akd.2015.5849

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Abstract

Objective: Adiponectin is a unique anti-atherogenic adipocytokine. Regulation of adiponectin secretion is dysfunctional in cardiovascular diseases. The current trial study assessed the effects of omega-3 fatty acids with or without vitamin E on adiponectin gene expression in peripheral blood mononuclear cells and serum adiponectin and adipocyte fatty acid-binding protein (A-FABP; also called ap2 and FABP4) levels in patients with coronary artery disease (CAD). Methods: This randomized, double-blind, placebo-controlled trial included 67 male patients with CAD. First of the four group of participants received 4 g/day omega-3 fatty acids plus 400 IU/day vitamin E (OE), second group 4 g/day omega-3 fatty acids plus vitamin E placebo (OP), or both omega-3 fatty acid and vitamin E placebos (PP) for 8 weeks. Adiponectin gene expression and serum adiponectin and FABP4 levels were evaluated. Results: The combination of omega-3 fatty acids and vitamin E in patients with CAD affected their serum adiponectin and FABP4 levels and the adiponectin/FABP4 ratio significantly. In the OP group, serum adiponectin levels did not change significantly. Consumption of omega-3 fatty acids with and without vitamin E had no significant effect on adiponectin gene expression. Conclusion: Omega-3 fatty acids with or without vitamin E improve adiponectin levels in patients, without any significant changes in adiponectin gene expression. This nutritional intervention may prevent complications in patients with CAD because of increased adiponectin levels.

Keywords: coronary artery disease, omega-3 fatty acids, vitamin E, adiponectin, adipocyte fatty acid-binding protein