, Mehmet Salıh Aydın 1
, Yasemin Hacanlı 1
, Nazım Kankılıç 1
, İsmail Koyuncu 2
, Muhammet Emin Güldür 3
, Ebru Temiz 2
, Yusuf Çakmak 4
, Kadir Eği 5
, Reşat Dikme 6
, Mahmut Padak 6
2Department of Medical Biochemistry, Faculty of Medicine, Harran University, Şanlıurfa, Türkiye
3Department of Pathology, Faculty of Medicine, Islamic Science and Technology University, Gaziantep, Türkiye
4Harran University, Animal Experiment Application and Research Center (HDAM), Şanlıurfa, Türkiye
5Harran University, Medical Promotion and Marketing Program, Health Services Vocational School, Şanlıurfa, Türkiye
6Harran University, Vocational School of Health Services, Perfusion Techniques Program Şanlıurfa, Türkiye
Abstract
Background: Pistacia vera L. (green pistachio) has been shown to increase antioxidant capacity and protect against cardiovascular diseases and cancer. This study investigated the protective effect of the Pistacia vera L. hull in rats with experimental cardiac damage induced by doxorubicin.
Methods: Sixty adult Wistar albino rats were randomly divided into 5 groups (n = 12). Sham, doxorubicin, doxorubicin + Pistacia vera L. extract 50 mg/kg, doxorubicin + Pistacia vera L. extract 100 mg/kg, and Pistacia vera L. extract 100 mg/kg. Biochemistry parameters, total antioxidant status, total oxidant status, oxidative stress index, 8-hydroxydeoxy guanosine, and caspase 3/7 values were measured in serum samples. Excised heart tissues were examined histopathologically.
Results: The groups were statistically significantly different in 8hydroxydeoxy guanosine, caspase 3/7, total antioxidant status, total oxidant status, oxidative stress index, and basal biochemical parameter values (P <.05, P <.001). In group II, 8-hydroxydeoxy guanosine, caspase 3/7, and total oxidant status values increased while the total antioxidant status value decreased (P <.001). In the treatment groups (group III and group IV), 8-hydroxydeoxy guano sine and caspase 3/7 values decreased compared to group II (P < .001). While total oxidant status and oxidative stress index values decreased in the treatment groups, total antioxidant status values increased (P <.001). The histopathological examination of the heart revealed fewer areas of focal necrosis in the treatment groups compared to group II.
Conclusion: In this study, the cardioprotective effect of Pistacia vera L. hull extract was investigated in vivo. It was shown that Pistacia vera L. hull extract reduced apoptosis and deoxyribonucleic acid damage in the face of cardiac damage and had antioxidant activity. Future studies will increase our knowledge on this subject.