Objective: This study was performed to investigate the functions of mRNA, long non-coding RNA (lncRNA), and circular RNA (circRNA) on paroxysmal and persistent atrial fibrillation (ParoAF and PersAF) patients.
Methods: Nine left atrial appendage (LAA) tissues were collected from patients with AF (ParoAF patients n = 3 and PersAF patients n = 3) and donors (n = 3). Genes and circRNAs were identified by per kilobase per million reads (RPKM) and number of circular reads/number of mapped reads/read length (SRPBM), respectively. Differentially expressed mRNAs (DE mRNAs), lncRNAs (DE lncRNAs), and circRNAs (DE circRNAs) were identified by | log2 (Fold Change) | ≥ 2 and P-value ≤ 0.05. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed. Protein-protein, mRNA-lncRNA, and circRNA-miRNA interaction networks were constructed. Moreover, the logistic analysis among AF and circRNAs was conducted.
Results: A total of 285 (116 up-regulated and 169 down-regulated) and 275 (110 up-regulated and 165 down-regulated) DE mRNAs, 575 (276 up-regulated and 299 down-regulated) and 583 (330 up-regulated and 253 down-regulated) DE lncRNAs, and 83 (48 up-regulated and 35 down-regulated) and 99 (58 up-regulated and 41 down-regulated) circRNAs were detected in ParoAF and PersAF compared with control, respectively. MAPK signal pathway and calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C) might participate in the AF occurrence by preventing atrial parasympathetic remodeling. Collagen, type I, alpha 1 (COL1A1) and COL1A2 mostly participated in the enriched GO and KEGG terms and connected with most of the DE mRNAs. The expression of chr10: 69902697|69948883 was a protective factor against PersAF after adjusting age (P = 0.022, 95% CI: 0.003-0.634).
Conclusion: We found some mRNAs, lncRNAs, circRNAs, and pathways play essential roles in AF pathogenesis and development. Moreover, one protective factor against PersAF was detected.