ISSN 2149-2263 | E-ISSN 2149-2271
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Volume : 25 Issue : 4 Year : 2021
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Integrative analysis reveals essential mRNA, long non-coding RNA (lncRNA), and circular RNA (circRNA) in paroxysmal and persistent atrial fibrillation patients [Anatol J Cardiol]
Anatol J Cardiol. Ahead of Print: AJC-57295 | DOI: 10.14744/AnatolJCardiol.2020.57295

Integrative analysis reveals essential mRNA, long non-coding RNA (lncRNA), and circular RNA (circRNA) in paroxysmal and persistent atrial fibrillation patients

Haoliang Sun1, Junjie Zhang2, Yongfeng Shao1
1The First Affiliated Hospital Of Nanjing Medical University
2Changzhou Wujin People’s Hospital

Objective: This study was performed to investigate the functions of mRNA, long non-coding RNA (lncRNA), and circular RNA (circRNA) on paroxysmal and persistent atrial fibrillation (ParoAF and PersAF) patients.
Methods: Nine left atrial appendage (LAA) tissues were collected from patients with AF (ParoAF patients n = 3 and PersAF patients n = 3) and donors (n = 3). Genes and circRNAs were identified by per kilobase per million reads (RPKM) and number of circular reads/number of mapped reads/read length (SRPBM), respectively. Differentially expressed mRNAs (DE mRNAs), lncRNAs (DE lncRNAs), and circRNAs (DE circRNAs) were identified by | log2 (Fold Change) | ≥ 2 and P-value ≤ 0.05. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed. Protein-protein, mRNA-lncRNA, and circRNA-miRNA interaction networks were constructed. Moreover, the logistic analysis among AF and circRNAs was conducted.
Results: A total of 285 (116 up-regulated and 169 down-regulated) and 275 (110 up-regulated and 165 down-regulated) DE mRNAs, 575 (276 up-regulated and 299 down-regulated) and 583 (330 up-regulated and 253 down-regulated) DE lncRNAs, and 83 (48 up-regulated and 35 down-regulated) and 99 (58 up-regulated and 41 down-regulated) circRNAs were detected in ParoAF and PersAF compared with control, respectively. MAPK signal pathway and calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C) might participate in the AF occurrence by preventing atrial parasympathetic remodeling. Collagen, type I, alpha 1 (COL1A1) and COL1A2 mostly participated in the enriched GO and KEGG terms and connected with most of the DE mRNAs. The expression of chr10: 69902697|69948883 was a protective factor against PersAF after adjusting age (P = 0.022, 95% CI: 0.003-0.634).
Conclusion: We found some mRNAs, lncRNAs, circRNAs, and pathways play essential roles in AF pathogenesis and development. Moreover, one protective factor against PersAF was detected.

Keywords: Atrial fibrillation, RNA-Seq, Pathway, mRNA, lncRNA, circRNA



Corresponding Author: Yongfeng Shao, China