ISSN 2149-2263 | E-ISSN 2149-2271
The Anatolian Journal of Cardiology
mRNA PGC-1α levels in blood samples reliably correlates with its myocardial expression: study in patients undergoing cardiac surgery [Anatol J Cardiol]
Anatol J Cardiol. 2016; 16(8): 622-629 | DOI: 10.5152/AnatolJCardiol.2015.6466

mRNA PGC-1α levels in blood samples reliably correlates with its myocardial expression: study in patients undergoing cardiac surgery

Oscar Fabregat-andres1, Federico Paredes1, María Monsalve1, Javier Milara1, Francisco Ridocci-soriano1, Sonia Gonzalez-hervas1, Armando Mena1, Lorenzo Facila1, Fernando Hornero1, Salvador Morell1, Juan Martinez-leon1, Julio Cortijo2
1Cardiovascular Institute, General University Hospital from Valencia- Spain
2Research Foundation, General University Hospital from Valencia- Spain

Objective: Peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) is a transcriptional coactivator that has been proposed to play a protective role in mouse models of cardiac ischemia and heart failure, suggesting that PGC-1α could be relevant as a prognostic marker. Our previous studies showed that the estimation of peripheral mRNA PGC-1α expression was feasible and that its induction correlated with the extent of myocardial necrosis and left ventricular remodeling in patients with myocardial infarction. In this study, we sought to determine if the myocardial and peripheral expressions of PGC-1α are well correlated and to analyze the variability of PGC-1α expression depending on the prevalence of some metabolic disorders.
Methods: This was a cohort of 35 consecutive stable heart failure patients with severe aortic stenosis who underwent an elective aortic valve replacement surgery. mRNA PGC-1α expression was simultaneously determined from myocardial biopsy specimens and blood samples obtained during surgery by quantitative PCR, and a correlation between samples was made using the Kappa index. Patients were divided into two groups according to the detection of baseline expression levels of PGC-1α in blood samples, and comparisons between both groups were made by chi-square test or unpaired Student’s t-test as appropriate.
Results: Based on myocardial biopsies, we found that mRNA PGC-1α expression in blood samples showed a statistically significant correlation with myocardial expression (Kappa index 0.66, p<0.001). The presence of higher systemic PGC-1α expression was associated with a greater expression of some target genes such as silent information regulator 2 homolog-1 (x-fold expression in blood samples: 4.43±5.22 vs. 1.09±0.14, p=0.044) and better antioxidant status in these patients (concentration of Trolox: 0.40±0.05 vs. 0.34±0.65, p=0.006).
Conclusions: Most patients with higher peripheral expression also had increased myocardial expression, so we conclude that the non-invasive estimation of mRNA PGC-1α expression from blood samples provides a good approach of the constitutive status of the mitochondrial protection system regulated by PGC-1α and that this could be used as prognostic indicator in cardiovascular disease. (Anatol J Cardiol 2016; 16: 000-00)

Keywords: peroxisome proliferator-activated receptor-γ, coactivator-1α, mRNA expression, cardiac surgery, prognosis marker

Oscar Fabregat-andres, Federico Paredes, María Monsalve, Javier Milara, Francisco Ridocci-soriano, Sonia Gonzalez-hervas, Armando Mena, Lorenzo Facila, Fernando Hornero, Salvador Morell, Juan Martinez-leon, Julio Cortijo. mRNA PGC-1α levels in blood samples reliably correlates with its myocardial expression: study in patients undergoing cardiac surgery. Anatol J Cardiol. 2016; 16(8): 622-629

Corresponding Author: Oscar Fabregat-andres, Spain
Manuscript Language: English

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