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Volume : 12 Issue : 8 Year : 2021
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The Anatolian Journal of Cardiology Bosentan ameliorates the expression of fibrotic related growth factors and collagen-1 in diabetic mice [Anatol J Cardiol]
Anatol J Cardiol. 2012; 12(8): 621-627 | DOI: 10.5152/akd.2012.213

Bosentan ameliorates the expression of fibrotic related growth factors and collagen-1 in diabetic mice

Bo Yang1, Min Li2, Zhen-Guo Shi3, Quan-Zhou Feng1
1Department of Cardiology, Chinese PLA General Hospital, Beijing-China
2Institute of Traditional Chinese Medicine, Chinese PLA General Hospital, Beijing-China
3Department of Pharmaceutical Administration, Chinese PLA General Hospital, Beijing-China

Objective: To investigate the potential beneficial effect of bosentan in ameliorating fibrotic agents in diabetic mice. Methods: Male 6-week old C57BL/6 mice were divided into 3 groups (N=20): Control group, diabetes mellitus (DM) group and DM-B group (diabetes with bosentan group). Streptozotocin (STZ) was injected as 200 mg/Kg for single dose, i.p. (intraperitoneal injection). Fasting blood glucose (FBG) was measured at 0-, 1-, 2-week after STZ injection to confirm that diabetes was induced in the mice. Bosentan (100mg/Kg) and placebo was given i.g. (intragastric administration) once a day immediately after STZ injection for 18 weeks. The mRNA expression of tissue growth factor beta (TGF-b), connective tissue growth factor (CTGF), vascular endothelial growth factor (VEGF) and collagen-1 were evaluated by RT-PCR and real-time PCR. Differences in the data between the groups were compared by Student t-test for independent samples. Results: After 18 weeks of diabetic situation, FBG of DM-B mice was significantly higher than that of control mice and was similar with that of DM mice (DM mice vs. control mice, p<0.001; DM-B vs. control mice, p<0.001; DM mice vs. DM-B mice, p>0.05). The cardiac VEGF mRNA (a potent angiogenic factor) level in DM-B mice was significantly higher than DM mice (p<0.01). The heart of DM-B mice also showed lower expression of fibrotic genes (TGF-b, CTGF and collagen-1) than DM mice (p<0.01). Conclusion: These findings indicate the potential usefulness of an ET receptor antagonist bosentan in the amelioration of fibrotic agents, which may promote tissue fibrosis. This may provide a promising therapeutical strategy for diabetic cardiac fibrosis.

Keywords: Bosentan, diabetes, fibrotic agents, cardiac fibrosis, endothelin blockade, tissue growth factor beta, connective tissue growth factor, vascular endothelial growth

Bo Yang, Min Li, Zhen-Guo Shi, Quan-Zhou Feng. Bosentan ameliorates the expression of fibrotic related growth factors and collagen-1 in diabetic mice. Anatol J Cardiol. 2012; 12(8): 621-627
Manuscript Language: English