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The Anatolian Journal of Cardiology Identifying the key microRNAs implicated in atrial fibrillation [Anatol J Cardiol]
Anatol J Cardiol. Ahead of Print: AJC-41625 | DOI: 10.14744/AnatolJCardiol.2020.41625

Identifying the key microRNAs implicated in atrial fibrillation

Yuejuan Cao, Li Cui
Department of Cardiology, Tianjin Union Medical Center

Background: To investigate the potential microRNA (miRNAs) having diagnostic value in atrial fibrillation (AF).
Methods: miRNA and mRNA expression profiling of atrial tissue of AF and healthy individuals were downloaded from public Gene Expression Omnibus database. The differentially expression miRNAs/mRNAs (DEMis/DEMs) were identified in AF compared with controls. Interaction network between DEMis and DMEs was constructed. The biological processes, molecular functions and signaling pathways of DEMs were enriched. The diagnostic value of candidate DECs among AF and healthy individuals were preliminarily evaluated in GSE101586, GSEE101684, GSE112214 datasets.
Results: total of 20 DEMis were identified in AF compared with normal controls, including 7 up-regulated and 13 down-regulated DEMis. Furthermore, 2307 DEMs were identified in AF. In DEMi-DEM interaction network, down-regulated miR-193b and up-regulated miR-16 interacted with the most targeted DEMs, which interacted with 72 and 65 targeted DEM, respectively. The targeted DEMs were significantly enriched in biological functions including programmed cell death, PI3K-Akt signaling pathway, mTOR signaling pathway, Hippo signaling pathway, HIF-1 signaling pathway and ErbB signaling pathway. Four out of 20 DEMis had potential value to distinguish AF patients from healthy individuals in GSE68475, GSE70887 and GSE28954 datasets, including miR-490-3p, miR-630, miR-146b-5p and miR-367. The area under the curve (AUC) of those four DEMis was 0.751, 0.719, 0.709 and 0.7, respectively.
Conclusions: DEMis might play key roles in AF progression through, mTOR signaling pathway and Hippo signaling pathway. miR-409-3p, miR-630, miR-146b-5p and miR-367 had potential diagnostic value to discriminate AF from controls in our work.

Keywords: atrial fibrillation, microRNA, diagnosis

Corresponding Author: Yuejuan Cao, China