ISSN 2149-2263 | E-ISSN 2149-2271
The Anatolian Journal of Cardiology
Leptin Accelerates Endothelial Wound Healing: Role of Endothelial Nitric Oxide Synthase Expression [Anatol J Cardiol]
Anatol J Cardiol. 2022; 26(10): 750-756 | DOI: 10.5152/AnatolJCardiol.2022.1607

Leptin Accelerates Endothelial Wound Healing: Role of Endothelial Nitric Oxide Synthase Expression

Gürkan Yiğittürk1, Melike Özgu&776;l Önal1, Ekin Subaşı2, Beyza Doğan2, Melih Türkmenoğlu2, Merve Kaplan2, Volkan Yaşar1, Hülya Elbe1, Feral Öztu&776;rk1
1Department of Histology and Embryology, Faculty of Medicine, Muğla Sıtkı Koçman University, Muğla, Turkey
2Phase 6, Faculty of Medicine, Muğla Sıtkı Koçman University, Muğla, Turkey

Background: The endothelium is crucial for the control of vascular homeostasis and plays a role in angiogenesis. Leptin, a protein released mainly by adipose tissue, plays a key role in the regulation of energy balance and angiogenesis. We aimed to investigate the changes of endothelial nitric oxide synthetase expression on human umbilical vein endothelial cells wound healing model after leptin treatment.

Methods: In this study, 5 groups were planned as Group 1: control (untreated), Group 2: treated with 0.1 ng/mL leptin, Group 3: treated with 1 ng/mL leptin, Group 4: treated with 10 ng/mL leptin, and Group 5: treated with 100 ng/mL leptin. Closure rates of wound areas were calculated by the Image J program after 24 hours of leptin treatment. The WST-1 assay was used to calculate the cell viability. Immunocytochemical analysis was performed for endothelial nitric oxide synthase expression and H-Score was calculated.

Results: The closure rates of wound areas were calculated as 80.24%, 89.73%, 87.40%, 90.73%, and 93.70%, respectively. When all groups treated with leptin were compared with the control group, there was a statistically significant difference (P <.05). The WST-1 results showed that the most increasing levels of viable cells were found in the groups treated with 0.1 ng/mL leptin and 100 ng/mL leptin when compared to the control group. H-Score values of each group were calculated as 284.8 ± 15.22, 288.6 ± 8.41, 291 ± 8.16, 295.2 ± 11.60, and 308.8 ± 4.32, respectively. The difference between the control group and the group treated with 100 ng/mL leptin was statistically significant (P <.05).

Conclusions: Endothelial nitric oxide synthase expression in human umbilical vein endothelial cells increased depending on the leptin dose and the highest increase was in the group treated with 100 ng/mL leptin.

Keywords: Leptin, eNOS, endothelial wound healing

Gürkan Yiğittürk, Melike Özgu&776;l Önal, Ekin Subaşı, Beyza Doğan, Melih Türkmenoğlu, Merve Kaplan, Volkan Yaşar, Hülya Elbe, Feral Öztu&776;rk. Leptin Accelerates Endothelial Wound Healing: Role of Endothelial Nitric Oxide Synthase Expression. Anatol J Cardiol. 2022; 26(10): 750-756

Corresponding Author: Gürkan Yiğittürk
Manuscript Language: English


Journal Metrics

Journal Citation Indicator: 0.18
CiteScore: 1.1
Source Normalized Impact
per Paper:
0.22
SCImago Journal Rank: 0.348

Quick Search



Copyright © 2024 The Anatolian Journal of Cardiology



Kare Publishing is a subsidiary of Kare Media.