ISSN 2149-2263 | E-ISSN 2149-2271
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The Anatolian Journal of Cardiology The inhibition of miRNA-130a protects atherosclerosis through suppression of inflammation by proliferator-activated†receptor†γ (PPARγ) expression [Anatol J Cardiol]
Anatol J Cardiol. Ahead of Print: AJC-56721

The inhibition of miRNA-130a protects atherosclerosis through suppression of inflammation by proliferator-activated†receptor†γ (PPARγ) expression

Youshan Li, Fengtong Liu, Yali Liu, Yuqing Du
Department Of Peripheral Vascular, Dongzhimen Hospital, Beijing University Of Chinese Medicine, Beijing 100700, China

Objective: The current study was designed to evaluate the mechanism of atherosclerosis and protect against atherosclerosis.
Methods: C57BL/6 mice (n = 6) were fed normal diet for 12 weeks as sham group. Apoe-/- mice were fed the high-fat diet for 12 weeks as AS model group. Serum samples or cell was used to measure the expression of TNF-NF-el group. Serum samples or cell was usedMiRNA expression profiles was determined using the edgeR tool from Bioconductor. Human umbilical vein endothelial cells (HUVECs) of vitro model was made. Western Blot analysis was used to assess the expression of PPARγPARl was made. Western Blot analysis was used to assess the expression of PPARγ and NF-κB.
Results: Our results suggested that miRNA-130a expression was up-regulated in atherosclerotic mice. Additionally, over-expression of miRNA-130a would promote inflammation (TNF-α, IL-1β, IL-6 and IL-8) in the in vitro model. By contrast, down-regulation of miRNA-130a would reduce inflammation (TNF-α, IL-1β, IL-6 and IL-8) in the in vitro model. Furthermore, over-expression of miRNA-130a could also suppress the protein expression of proliferator-activated†receptor†γ (PPARγ), while induce that of NF-κB in the in vitro model. However, down-regulation of miRNA-130a would induce the protein expression of PPARγ whereas suppress that of NF-κB in the in vitro model. Moreover, activation of PPARγ would reduce the effects of miRNA-130a on the inflammation in the in vitro model.
Conclusion: Taken together, these results suggest that miRNA-130a suppression can protect against in atherosclerosis through inhibiting inflammation by regulating the PPARγ/ NF-κB expression.

Keywords: miRNA-130a, atherosclerosis, inflammation, PPARγ,, NF-κB



Corresponding Author: Youshan Li, China